The goal is to get the X(um) and Y(um) values as small and as close together as possible.
- Turn the fast scan OFF
- Set the integration time to x1 (x1 = exposure 1; x2 = exposure 2; etc.).
- You must calibrate each integration time separately. If you will not use exposures beyond 1, which is almost always the case, you don't need calibrate x2, x3 etc. Also if you will not use on or more of the individual image sizes (768/580; 1024/774; 1928/1450) you need not calibrate them. In practice you should really only have to calibrate one settings: Integration time set to x1; Image format 1928/1450 (these settings will provide the highest quality images).
- Click "All" and ensure that all checkboxes are selected
- Set the threshold.
- The lower the threshold the better
- Manufacturer suggests 0.3
- We suggest 0.11, lower is possible but difficult to achieve in the setup at the Chalcidoidea lab
- Set the number of iterations.
- It should not take more than 20 iterations to get the threshold to 0.11 low. If it does, the lighting arms position needs to be adjusted and the power of the light sources may need to be increased or decreased. If when calibrating either the x or y is consistently much higher than the other then the camera will not calibrate successfully this time- abort the calibration and reset the lighting.
- Click on the "Start Calibration" button.
- Click "Ok" on when asked to setup the slightly focused checkerboard (done earlier)
- Wait for calibration to complete
- Hints:
- When the lighting is set up properly the camera will calibrate very quickly, you should know in 4-5 iterations if a successful calibration will be achieved. You can determined this by watching the change in the x/y values while the calibration is running. If the values "hover" near your threshold they will reach it. If your threshold is at 0.1 and the values consistently are around 0.3 you will likely not calibrate successfully this round, adjust the lighting and retry (remember to use the condensers!!)
- Sometimes
it is difficult to calibrate the Prescan. It should be easily done with
the correct light setting but if it will consistently not calibrate then
deselect and calibrate the rest of the
- The camera should now be calibrated.
- Double check the "Configure" dialog off of the main dialog:
- The matrix should be Ps3200k.mtx for halogen light sources
- Check: Colour Balance; Gradation Curves; Brightness & Contrast; and Scale Correction
- Uncheck: All other check boxes
- Choose RGB (colour) or Monochrome (Black & White)
- Color values should be set to 0-100%
- make sure you have chosen the correct Image Format (almost always 1928*1450)
- gain = 0; exposure =1 (to start with)